Effects of temperature on the early development of carpospores of Ahnfeltiopsis flabelliformis (Gigartinales, Rhodophyta)
Zhao, Fengjuan; Zhao, Ziguo; Liu, Jidong
Morphological and culture studies of juvenile germlings derived from carpospores of Ahnfeltiopsis flabelliformis (Harv.) Masuda were carried out at different temperature regimens (10, 15, 20, 25°C) with 32 μmol photons m-2s-1 and 10:14 (L/D) photoperiod. Our results showed that the development of carpospores can be divided into three stages: division stage, discoid crust stage and juvenile germling stage. Filaments and the coalescence of larger discoid crusts were observed during the discoid crust stage. Two up to more than five of discoid crusts may coalesce into a single larger discoid crust, which usually then developed into juvenile germlings. The temperature significantly influenced carpospore germination and the discoid crust formation. Carpospores germinated at a range of 15–25°C under 32 μmol photons m-2s-1, and the optimal condition was at 20°C and 25°C. For the growth of discoid crusts, maximum growth occurred at 25°C. Our results will increase our knowledge on the life history of sexual reproduction of A. flabelliformis, which should be helpful to improve germling nursery of this alga.