Original paper

Genetic variation of 15 autosomal STRs in a population sample of Bedouins residing in the area of the Fourth Nile Cataract, Sudan

Ossowski, Andrzej; Piatek, Jaroslaw; Parafiniuk, Miroslaw; Pudlo, Aleksandra; Pepinski, Witold; Skawronska, Malgorzata; Szeremeta, Michal; Niemcunowicz-Janica, Anna; Soltyszewski, Ireneusz

Anthropologischer Anzeiger Volume 74 No. 4 (2017), p. 263 - 268

published: Dec 1, 2017
published online: Aug 17, 2017
manuscript accepted: Jun 12, 2017
manuscript revision received: May 21, 2017
manuscript revision requested: Apr 24, 2017
manuscript received: Oct 18, 2016

BibTeX file

ArtNo. ESP140007404000, Price: 29.00 €

Download preview PDF Buy as PDF

Abstract

Abstract The purpose of the paper was to report allelic frequencies of 15 autosomal STR markers (AmpFlSTR NGM PCR Amplification Kit) for Bedouin inhabitants in the area of the Fourth Nile Cataract in Sudan, and compute commonly used population and forensic biostatistical parameters. Buccal swabs were collected from 117 unrelated individuals. DNA was extracted using DNA QIAamp® DNA Mini Kit, and quantitated with Quantifiler Human Quantification Kit in a 7500 Real-Time PCR System. Amplification of 15 AmpFlSTR NGM PCR Kit loci was performed in PCR System 9700. Electrophoresis and typing were performed in 3130 Genetic Analyzer. Arlequin v3.5 software and PowerStats v1.2 spreadsheet were used for statistical calculations. The STR frequency distributions showed no deviations from HWE. The combined values of Matching Probability and Power of Exclusion are 1.77 × 10–18 and 0.9999996, respectively. The average observed heterozygosity over 15 loci is 0.8069. Five different allelic microvariants were found. A significant linkage disequilibrium was observed in five pairs of loci. A 15 STR population database has been established for Sudanese Bedouins. The systems studied have been shown to be useful tool for personal identification in this population.

Keywords

Autosomal STRsPopulation geneticsBedouinsSudan