Original paper

The cultivation of Phaeodactylum tricornutum in crossed gradients of temperature and light

Kvíderová, Jana; Lukavský, Jaromír

Abstract

Phaeodactylum tricornutum Bohlin, is one of the most important species in the algal biotests. The cultivation of this species in the crossed gradients evaluated the dependence of the growth rates and morphology on particular combinations of environmental factors (temperature, light). The main advantage of this method is the simplicity and quickness of obtaining data from different conditions.The results of the cultivation of Phaeodactylum tricornutum, in the crossed gradients, showed that maximal growth rates occurred at temperatures between 15 and 23 °C and at irradiance between 15 and 125 μmol.m−2.s−1). There were not observed differences in morphology of the alga cultivated at different combinations of growth conditions.The conversion curves were calculated for three cultures of Phaeodactylum tricornutum (two old cultures and one young one) and for four different ways of measurements of optical density at 750 nm (in immunological plates, culture wells, Petri dishes and 20 ml cultivation flasks).

Keywords

phaeodactylum tricornutumcrossed gradientscultivationlighttemperaturegrowth rategrowth optimaconversion curves