Original paper

Toxicity of metals, Al, Cd, Co, Cr, Cu, Fe, Ni, Pb and Zn on microalgae, using microplate bioassay 1: Chlorella kessleri, Scenedesmus quadricauda, Sc. subspicatus and Raphidocelis subcapitata (Selenastrum capricornutum)

Lukavský, Jaromír Furnadjieva; Cepák, Vladislav


Toxicities of Al, Cd, Co, Cr, Cu, Fe, Ni, Pb and Zn were tested using growth miniaturized algal bioassay with Chlorella kessleri, Raphidocelis subcapitata (= Selenastrum capricornutum), Scenedesmus quadricauda and Scenedesmus subspicatus. Toxicity hierarchy for all organisms, according to their mean values is: Cd> Co> Cr> Cu> Pb> Ni> Zn> Al> Fe. The sensitivity hierarchy is: Raphidocelis > Scenedesmus subspicatus > Chlorella > Scenedesmus quadricauda. The most toxic element was Cd, which was the first in toxicity hierarchy for all tested organisms. Cd has a gradual toxic curve, and has showed no stimulation, similar to Pb. Scenedesmus quadricauda showed a peak shape of growth curve with stationary phase about 300 h. Another algae showed prolonged and approximately stabile stationary phase in the period of 300-700 h, under PhAR 180 μE.m−2.s−1, (40 W.m−2) 30 °C, and CO2 supply ca 2 % (v/v).


heavy metalsalcdcocrcufenipbzntoxicityec50inhibitionstimulationgrowth bioassayimmunological platesdry weightoptical densityalgaechlorella kessleriraphidocelis subcapitata (selenastrum capricornutuscenedesmus quadricaudascenedesmus subspicatus