Original paper

Extracellular polar organic substances in cultures of the green alga Scenedesmus

Kambourova, Roumena; Petkov, Georgi; Bankova, Vassya


The organic excretion of non-sterile monoalgal cultures of Scenedesmus incrassatulus grown in fresh and Black Sea water were investigated using gas chromatography/mass spectrometry. Lactic acid predominated, oxalic, hydroxybutiric, succinic acids, and some organic phosphates were also found. Dehydroabietic, 2-hydroxy-3-(4-hydroxyphenyl)-propanoic, N,N-Dimethylglycine, N,N-dimethylisopropenylglycine were identified, they are new found substances in algae. Algal cultures at exponential growth phase, which was characterized with high density around 4 g.dm−3, excrete relatively less organic matter than the phytoplankton in natural ponds or starving algal cultures in model experiments.


algaen,n-dimethylglycineextracellularlactic acidscenedesmus