Original paper

Measurement of phosphomonoesterase activity in wetland sediments - a sensitive method using HPLC and UV detection

Kang, Hojeong; Freeman, Chris

Archiv für Hydrobiologie Volume 140 Number 3 (1997), p. 411 - 417

15 references

published: Nov 10, 1997

DOI: 10.1127/archiv-hydrobiol/140/1997/411

BibTeX file

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We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800 μM of substrate concentration.