Measurement of phosphomonoesterase activity in wetland sediments - a sensitive method using HPLC and UV detection
Kang, Hojeong; Freeman, Chris
Archiv für Hydrobiologie Volume 140 Number 3 (1997), p. 411 - 417
published: Nov 10, 1997
ArtNo. ESP141014003008, Price: 29.00 €
We have modified the method for the assay of phosphomonoesterase using methylumbelliferyl substrates in wetland sediments, by adopting HPLC with UV detection. Separation of the enzymic products from the sample matrix overcomes the problem caused by quenching interference of phenolics in the conventional fluorometric assay, and yet does not require an expensive fluorometric HPLC detector. Phosphomonoesterase activity measured by this method, showed linearity over a 90-minute incubation period and substrate saturation at 800 μM of substrate concentration.