Characterization and diversity of microcystins in natural blooms and strains of the genera Microcystis and Planktothrix from German freshwaters
Fastner, J.; Erhard, M.; Carmichael, W. W.; Sun, F.; Rinehart, K. L.; Rönicke, H.; Chorus, I.
Archiv für Hydrobiologie Volume 145 Number 2 (1999), p. 147 - 163
published: May 27, 1999
ArtNo. ESP141014502007, Price: 29.00 €
Fifteen microcystins (MC) were isolated from field samples dominated by Microcystis spp., Planktothrix agardhii or Planktothrix rubescens using reversed phase high-performance liquid chromatography (HPLC). Characterization of microcystins was performed with amino acid analysis followed by fast atom bombardment mass spectrometry (FABMS), and/or matrix assisted laser desorption ionization mass spectrometry (MALDI-TOF MS). In blooms of Microcystis spp. major microcystins were usually MC-RR, MC-YR and MC-LR, while in one strain [D-Asp3] MC-LR and [D-Asp3] MC-RR were also quantitatively abundant. The main microcystins found in field samples of Planktothrix agardhii were [D-Asp3] MC-RR followed by [D-Asp3