Original paper

A new preparation technique of daphnids for Scanning Electron Microscopy using hexamethyldisilazane

Laforsch, Christian; Tollrian, Ralph

Archiv für Hydrobiologie - Hauptbände Volume 149 Number 4 (2000), p. 587 - 596

27 references

published: Nov 24, 2000

DOI: 10.1127/archiv-hydrobiol/149/2000/587

BibTeX file

ArtNo. ESP141014904003, Price: 29.00 €

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Abstract

SEM requires special preparation techniques to avoid artifacts, especially in aquatic organisms. Not all organisms can be prepared equally well for SEM. For example in Daphnia, preparation causes major shrinkage of the carapace in most species. We present a new preparation technique for Daphnia using hexamethyldisilazane (HMDS), which avoids these problems. We compared this method with the common critical point drying technique (CPO). The routinely used CPO caused shrinkage in the daphnids, predominantly in the region of the neck and the brood chamber. In contrast, all tested Daphnia species immersed in hexamethyldisilazane (HMOS) and dried slowly overnight in a vacuum desiccator revealed an excellent surface. The good results, low costs and easy operation without the requirement of special equipment favor HMOS as drying agent for SEM investigations in daphnids.

Keywords

Daphniapreparation techniqueshrinkagesurface structurescritical point drying technique (CPO)HMDS