Microbial phosphatase in biofilms: A comparison of whole community enzyme activity and individual bacterial cell-surface phosphatase expression
Espeland, Eric M.; Francoeur, Steven N.; Wetzel, Robert G.
Archiv für Hydrobiologie Volume 153 Number 4 (2002), p. 581 - 593
published: Apr 9, 2002
ArtNo. ESP141015304003, Price: 29.00 €
Phosphatase of natural photosynthetic biofilms was examined to elucidate the relationship between whole-community (algal + bacterial + protistan) phosphatase activity and individual bacterial cell phosphatase statement under incipient phosphorus deficiency. Additionally, several fluorescent dyes and substrates were used to enumerate phosphatase-producing and membrane-intact "live" cells. Phosphorus deficiency caused increased production of free dissolved phosphatase (p < 0.05) without significant changes in surface-bound phosphatase activity. the proportion of bacteria displaying phosphatase activity increased 21 % over time but was not significantly correlated to total phosphatase (rs = -0.167, p = 0.424) or surface-bound activity (rs = -0.003, p = 0.988). "Live" bacterial cells comprised a smaller proportion of the total bacterial cell counts than to phosphatase-active cells, indicating that surface-bound phosphatase remained active following cell senescence.