Original paper

Quantification of individual native biocolloids in natural waters: assessing indicators of aquatic events, by using transmission electron microscopy with a modified approach to image analysis

Leppard, Gary G.; Arsenault, A. Larry


Colloids and colloid aggregates are found abundantly in riverine, lacustrine and marine ecosystems where they play important roles in the transport and transformations of contaminants and nutrients. To understand colloid impacts better, new methodology had been created to quantify the native colloid burdens of diverse natural waters. As progress was achieved by the resultant bulk characterizations, there arose a need to quantify individually the major colloid species in a water sample; the individual species have different activities and thus affect biogeochemical processes differentially. Analytical electron microscopies, applied correlatively, can characterize individual colloids. This paper describes the use of transmission electron microscopy, coupled with a novel image analysis approach, to yield quantitative information on the ratios of abundant colloid species present in a given water sample.


colloid quantificationtransmission electron microscopyimage analysisfibrils.