Original paper

CO2 partial pressure pH relationships in the medium and relevance to CO2 mass balance in outdoor open thin-layer Arthrospira (Spirulina) cultures

Lívanský, Karel; Doucha, Jiří; Hu, Hongjun; Li, Yeguang

Archiv für Hydrobiologie Volume 165 Number 3 (2006), p. 365 - 381

published: Apr 21, 2006

DOI: 10.1127/0003-9136/2006/0165-0365

BibTeX file

ArtNo. ESP141016573005, Price: 29.00 €

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A mathematical formula was derived for pCO2 (partial pressure of dissolved carbon dioxide) in culture medium as a function of pH. The formula was verified in laboratory experiments with desorption of CO2 from the medium into air which had been bubbled through a medium commonly used (Zarrouk's recipe) for cultivation of the microalga Arthrospira (Spirulina). Concentration of sodium bicarbonate in the medium was 9 and 16.8 g l−1, respectively, at temperatures of 30 and 40°C. The overall volumetric mass transfer coefficient KL a/V for CO2 transfer from the medium into the gas phase increased about 2 % per 1°C increase of temperature and was not influenced by bicarbonate concentration in the medium. Mass balance of CO2 in a suspension of Spirulina flowing in a layer of 8 mm thickness down an inclined cultivation surface was formulated and investigated theoretically, taking into account CO2 consumption by the alga and CO2 mass transfer from the suspension into the atmosphere. Calculations suggested that dissolved free (not chemically bound) carbon dioxide and concentration of bicarbonate ions in the suspension are practically in mutual chemical equilibrium. Mass flux of carbon dioxide from the suspension into the atmosphere is enhanced at increased suspension pH as a result of CO2 formation from bicarbonate ions.


mediumcarbon dioxideequilibriumphkineticsdiffusionmass balancearthrospira (spirulina)open thin-layer culture