Lipovitellins and Amino Acid Pools during Embryogenesis of the Camel Tick, Hyalomma dromedarii (Acarina: Ixodidae)
Hamed, Ragaa R.; Fahmy, Afaf S.; Kamel, Mamdouh Y.
Two vitellins, A and B, are isolated and purified to homogenity from Hyalomma dromedarii (Koch 1844) oviposited eggs. Both A and B are identified as a haemoglycolipoproteins. UV-visible spectra of A and B indicate the presence of haeme moiety. The haeme moiety is protohaemin. Total carbohydrate and lipid content are found to be 9.78, 12.2% and 4.5, 20 for A and B respectively. - Amino acid analysis of A and B and free amino acids indicates that alanine, valine, leucine and lysine are the major constituents. Total free amino acid pool concentration decreases during embryogenesis. Amino acid concentrations can be separated into 2 groups according to their behaviour during embryogenesis. In the first group, there is a sharp decrease in early stages of embryogenesis (3-9 days) and increase in late stages and larvae. In the second group, there is a continuous decrease starting in the early stages and continuing till hatching.