Association of Different Endosymbionts with the Whitefly species Bemisia tabaci and Trialeurodes vaporariorum (Sternorrhyncha: Aleyrodidae)
Tan, Zhou-Jin; Xie, Bing-Yan; Xiao, Qi-Ming; Yang, Yu-Hong; Wan, Fang-Hao; Huang, Shi-Wen
The host range of Bemisia tabaci (Westwood 1856) and Trialeurodes vaporariorum (Gennadius 1889) and their kind of host damage are reported briefly. So far, the bacterial symbionts, associated with the biological characters of whitefly species, and their particular influence on the evolution of the whitefly host, are not yet studied sufficiently. Here, the composition and genomes of prokaryotic symbionts associated with the whitefly species B tabaci (B biotype) and T vaporariorum were examined from material collected on corresponding host plant species and from the same geographical locations. The ultrastructures of the endosymbionts in B tabaci and T vaporariorum were examined by using transmission electron microscopy: There are differences in morphology and relative number of endosymbionts between B tabaci and T vaporariorum. B tabaci contains two morphological types of microorganisms housed within the mycetocyte cells, but T vaporariorum contains only one morphological type of microorganisms housed within the mycetocyte cells. The 16S rDNA and groEL of the endosymbionts in B tabaci and T vaporariorum were obtained by polymerase chain reaction (PCR) amplification. The 16S rDNAs and groEL of the endosymbionts in B tabaci and T vaporariorum are found to be different: Primary endosymbionts in B tabaci are Proteobacteria: Gammaproteobacteria: Oceanospirillales: Halomonadaceae: Zymobacter-group: Candidatus portiera aleyrodidarum, and secondary endosymbionts in B tabaci are Proteobacteria: Gammaproteobacteria: Enterobacteriales: Enterobacteriaceae. The endosymbionts have important effects on B tabaci associated with its ecological distribution and the plant viral disease transmission. GroEL is very conservative. There is little difference among groEL from endosymbionts of B tabaci from different geographical origin. Amplification and comparison of 16S rDNA sequences revealed that a primary-like endosymbiont is associated with all whitefly samples examined.