Identification of Genes Expressed in Response to Dengue-2 Virus infection of Culex pipiens quinquefasciatus (Diptera: Culicidae)
[Feststellung von Genen, die infolge von Dengue-2 Virusinfektionen bei Culex pipiens quinquefasciatus zum Ausdruck kommen (Diptera: Culicidae)]
Yang, F; Guo, X; Dong, Y; Zhao, T; Lu, B
To reveal expression of related genes of Culex pipiens quinquefasciatus (Say 1982): in response to dengue-2 virus infection, differential display reverse-transcription PCR [DDRT-PCR] was used to identify molecular genetic changes in C pipiens quinquefasciatus after virus infection for 24 hs. Virus was isolated in C6/36 cells from mosquitoes after 24 hs infection. Seven differentially expressed cDNA bands were identified in susceptible and refractory mosquitoes. Six of them were up-regulated and one was down-regulated following infection. Sequence comparison the GenBank showed that a high expressed gene fragment named as CD16A. The nucleotide similarity of CD16A with cDNA of ribosomal protein S29 of Drosophila melanogaster and Mus musculus was 85% and 79%, respectively. The others were unknown gene fragments. Thus the fragment of CD16A should be the ribosomal protein S29 gene of Cx pipiens quinquefasciatus. The GenBank accession number is AF 266749. The here presented findings may suggest that ribosomal protein S29 gene was overexpressed in Cx pipiens quinquefasciatus indicating the restriction in host cell transcription. The result would be significant for understanding the mechanism of refractory in Cx pipiens quinquefasciatus to infection with dengue virus.