Original paper

A new species of Phormidium (Cyanobacteria, Oscillatoriales) from three Greek Caves: morphological and molecular analysis

Lamprinou, V.; Skaraki, K.; Kotoulas, G.; Anagnostidis, K.; Economou-Amilli, A.; Pantazidou, A.


Specimens of cyanobacteria were collected from three geographically distant caves in Greece, and an interesting species of the Oscillatoriales was isolated and analyzed. Morphological characters were analyzed from fresh and cultured material using LM, SEM and TEM; moreover, molecular analysis based on the 16 S rRNA sequence was applied. This cyanobacterium bears a characteristic blackish, stratified, mucilaginous sheath and it is characterized by entangled filaments, facultative presence of false branching, presence of necridia and peripheral arrangement of thylakoids. The autapomorpic blackish mucilaginous sheath and the pseudobranching filaments are unique morphological characters within the genus Phormidium, according to classical taxonomy. The 16 S rRNA sequence showed relatedness to Phormidium priestleyi ANT L52.6 with 97-98 % similarity, but without morphological support. Both phenotypic and genetic traits supported the designation of a new species, under the name Phormidium melanochroun Lamprinou & Pantazidou sp. nov.


16 s rrnacavescyanobacteriagreecelmoscillatorialesphormidium melanochroun sp. novsemtem