Original paper

Preliminary characterization of Typha latifolia and T. angustifolia from North America and Europe based on novel microsatellite markers identified through next-generation sequencing

Ciotir, Claudia; Dorken, Marcel; Freeland, Joanna

Abstract

Typha spp. comprise an increasingly aggressive species complex in North American wetlands. Complicated patterns of dispersal and hybridization challenge the elucidation of microevolutionary processes within this species complex, and therefore additional molecular markers are desirable. Microsatellite loci were identified in a broad-leaved cattail (Typha latifolia) using Roche 454 pyrosequencing technology. Nine of these loci were optimized, and all proved to be polymorphic within 76 samples of North American and European T. latifolia, having 2-17 alleles per locus. Seven of these loci were also amplified in 46 Typha angustifolia individuals from North America and Europe, and four were polymorphic, having 2-9 alleles per locus. Overall, allelic diversity was higher in T. latifolia than in T. angustifolia, a finding that contradicts earlier studies and identifies a need to investigate the potential influence of ascertainment bias. Multiple loci appeared to have species-specific and continent-specific alleles, although broader geographical studies are required for confirmation of these trends. This suite of markers, along with those previously characterized, will help to elucidate patterns of Typha dispersal within and between continents. In addition, they will facilitate studies of parentage, clonality, hybridization and admixture in this broadly distributed species complex.

Keywords

typha dispersalmicroevolutionary processespolymorphic molecular markersmicrosatellite lociparentageclonalityhybridizationadmixture