Morphological and molecular characterization of Phytopythium litorale and Pp. oedochilum from Iran
Chenari Bouket, Ali; Babai-Ahari, Asadollah; Arzanlou, Mahdi; Tojo, Motoaki
During a survey on the biodiversity of the genus Pythium in northwest region of Iran, three isolates belonging to the genus Phytopythium (Peronosporales) were isolated from the rhizosphere of Juncus sp. from the Myaneh region and Cirsium sp. from the Meshkin-Shahr region. Based on combination of cultural, morphological, cardinal growth rate and sequence data from ITS-rDNA, the isolate from Juncus sp. was identified as Pp. litorale and two isolates from Cirsium sp. were identified as Pp. oedochilum. Phylogenetic analyses of the ITS-rDNA sequences clustered our isolates with representative sequences for Pp. litorale and Pp. oedochilum isolates from GenBank in the Phytopythium clade. The genera Juncus and Cirsium are new hosts for Pp. litorale and Pp. oedochilum, respectively. Both species represent new records for the mycobiota of Iran. With this paper, we provide full illustration for these two species and further discuss their phylogeny and morphology with closely related species.