Molecular characterization and RFLP profile of an Inocybe (Inocybaceae, Agaricales) species isolated from Tlaxcala (Mexico): evidence for a new species in the subgenus Mallocybe
Meza-Meneses, Yeny; Galindo Flores, Gema; Martínez y Pérez, José Luis; Estrada-Torres, Arturo; Hernández-Cuevas, Laura V.; Alarcón, Alejandro; Montiel-González, Alba Mónica
Inocybe species are ectomycorrhizal fungi that have the potential to serve as inoculants for forestry plant species, especially in soils found in extreme conditions. Therefore, it is important to know its diversity, so the best adapted species for use in revegetation programs could be selected. The present research focused on the molecular characterization of one fungal morphotype in the Inocybe subgenus Mallocybe, a major fungal component in a pine plantation established on a disturbed area in the state of Tlaxcala, Mexico, as well as to generate a valid molecular marker for early and fast detection in field studies. The Internal Transcribed Spacer of the ribosomal DNA was amplified, sequenced and digested with four restriction enzymes in order to detect restriction fragment length polymorphisms (RFLPs). The sequence data of the product obtained has 807 base pairs (bp) and its BLAST analysis in the GenBank and UNITE databases resulted in an 84% similarity to Inocybe dulcamara f. pygmaea. The phylogenetic analysis indicates that the closest related species to the Mexican species is Inocybe terrigena. The RFLPs differ in important ways from the sequences of other species of Inocybe subgenus Mallocybe, and this supports their importance as tool for identification of the species in field samples. The evidence obtained in this research suggests that the Mexican species is different from other species in the subgenus Mallocybe previously studied since a molecular and phylogenetically point of views.