Delimitation of some European species of Calypogeia Raddi (Jungermanniales, Hepaticae) based on cytological characters and multienzyme phenotype
Buczkowska, Katarzyna; Odrzykoski, Ireneusz J.; Chudzinska, Ewa
Isoenzyme variants and oil body features were studied in 223 samples of six Calypogeia species from Poland (C. azurea, C. integristipula, C. neesiana, C. muelleriana, C. sphagnicola and C. suecica). Some representative samples were also examined for chromosome numbers. The distribution pattern of oil bodies- their presence or absence in some cells of the leaves and underleaves, as well as their colour and structure are proved to be particularly diagnostic. The multienzyme phenotype composed with isozyme variants of four enzymes is also diagnostic for all the studied species and highly correlated with oil body features. Thus isozyme phenotypes can be used as alternative diagnostic markers for prompt identification of living plants. The fixed heterozygosity pattern of two enzymes (TPI and GOT) found in the all studied samples of polyploid (n=18) C. azurea, C. muelleriana and C. sphagnicola may suggest an allopolyploid origin of these three species. All six studied samples of C. integristipula showed polyploid (n=18) chromosome numbers, new for this species.