Using fluorescently-labelled rRNA probes for hierarchical estimation of phytoplankton diversity a mini-review
Groben, RenéJohn; Eller, Gundula; Lange, Martin; Medlin, Linda K.
Molecular probes can be used to identify and characterize phytoplankton species by DNA dot blot or in situ hybridization with subsequent detection by fluorescence microscopy or flow cytometry. More or less conserved regions on the rRNA genes make it possible to develop probes that are specific for different taxonomic levels from higher groups, like eukaryotes, down to species and even strain levels. These hierarchical probes can be used to estimate biodiversity at a variety of taxonomic levels and are a valuable tool for analysing microalgae and answering ecological questions.