Original paper

DNA microchips for phytoplankton: The fluorescent wave of the future

Metfies, Katja; Medlin, Linda K.

Nova Hedwigia Band 79 Heft 1-2 (2004), p. 321 - 327

published: Aug 1, 2004

DOI: 10.1127/0029-5035/2004/0079-0321

BibTeX file

ArtNo. ESP050007901021, Price: 29.00 €

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The development of DNA-chip-technology has made it possible to analyse large numbers of probes in just one experiment. We show here the results of our first DNA-chip for the identification of phytoplankton. A set of previously published probes was chosen to be tested for their use on DNA-chips. Initially the probes were developed for other hybridization-techniques, such as dotblot-hybridization or FISH (fluorescent in situ hybridization). The probes target the 18S-rDNA of phytoplankton at the level of division or class. With the resulting DNA-chip, it was possible to identify specifically Prymnesiophyta, Chlorophyta, and the heterokont algal class Bolidophyceae. Our results indicate that DNA-chip-technology has great potential in phytoplankton analysis.