Original paper

Three Tuber ectomycorrhizae associated with Chinese Pine in North China – morpho-anatomical and molecular- phylogenetic analyses

Wei, Jie; Yan, Wei; Agerer, Reinhard

Abstract

Three anatomotypes of Tuber ectomycorrhizae on Chinese Pine (Pinus tabulaeformis) collected from North China are presented in this study. They all have pseudoparenchymatous outer mantle layers, but differ from each other in the shape of mantle cells and the presence of cystidia. "Pinirhiza pubulata" has angular cells in the outer mantle and abundant cystidia, "Pinirhiza puborchii" forms irregular epidermoid cells and few angular cells connected by septa in the outer mantle layer and abundant cystidia, whereas "Pinirhiza liaotongensis" has epidermoid cells in the outer mantle and lacks cystidia. The fungal partners of these three ectomycorrhizae are members of the genus Tuber as has been inferred by molecular phylogenetic analyses of the partial large subunit nuclear rDNA and by structural similarity to the ectomycorrhizae of this genus. "Pinirhiza liaotongensis" could be formed by a species of the Tuber liaotongense lineage, fungal partners of "Pinirhiza pubulata" and "Pinirhiza puborchii" could only be assigned to species groups according to the phylogenetic analyses of the internal-transcribed spacer (ITS) region. Phylogenetic analyses of the ITS region indicate that Tuber ECM with angular cells in the outer mantle is not monophyletic.

Keywords

anatomymolecular phylogenymorphologytuber ectomycorrhizae