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Chee-Jen Chen:

Morphological and molecular studies in the genus Tremella

1998. 225 pages, 174 figures, 25 tables, 14x22cm, 480 g
Language: English

(Bibliotheca Mycologica, Band 174)

ISBN 978-3-443-59076-5, paperback, price: 72.00 €

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Keywords

mycologyTremellamorphology

Contents

Synopsis top ↑

This study aims to investigate Taiwanese Tremella and related Sirobasidium species including type specimens. Additionally, other important herbarium material must be studied to understand the morphological differences which fructifications show in natural habitats. Investigating the association with hosts in symbiotic, parasitic and saprobic interactions will provide essential information for adequate culturing conditions.
In this work, comparative DNA analyses using partial 25 S and complete ITS sequences have been performed to estimate phylogenetical relationships of a number of taxa in the Tremellales. Comparing these results with conclusions of a thorough morphological and anatomical study, which are also presented here, seemed the best way to get an idea of the course of evolution in this interesting group of fungi.

Contents top ↑

1. Introduction 1
2. Materials and Methods 6
2.1. Morphology 6
2.1.1. List of specimens 6
2.1.2. Light microscopy 6
2.1.3. Transmission electron microscopy 6
2.1.3.1. Chemical preparation 7
2.1.3.2. Material preparation 7
2.2. rDNA sequences 8
2.2.1. Genomic DNA extraction and amplification 8
2.2.1.1. Chelex method 10
2.2.1.1.1. DNA extraction 10
2.2.1.1.2. PCR reaction after Chelex isolation 10
2.2.1.1.3. PCR program after Chelex isolation 10
2.2.1.2. Liquid nitrogen method 11
2.2.1.2.1. DNA extraction 11
2.2.1.2.2. PCR reaction after liquid nitrogen isolation 11
2.2.1.2.3. PCR program after liquid nitrogen isolation 12
2.2.2. Cyclic and fluorescence sequencing 12
2.2.3. Neighbor joining alignment 12
3. Results 13
3.1. Morphology 13
3.1.1. Mesenterica group 13
Tremella brasiliensis (Moller) Lloyd 15
Tremella cerebriformis C.-J. Chen 22
Tremella coalescens Olive 26
Tremella mesenterica Schw.: Fr. 29
Tremella taiwanensis C.-J. Chen 44
Tremella tropica C.-J. Chen 48
Tremellaflava C.-J. Chen 57
Tremella fuciformis Berkeley 67
Tremella resupinata C.-J. Chen 77
Tremella samoensis Lloyd 82
3.1.3. Indecorata group 85
Tremella exigua Desm. 88
Tremella globospora Reid 92
Tremella moriformis (Fr.) Smith ex Berk. 97
Tremella nivalis C.-J. Chen 101
Tremella foliacea Pers.: Fr. sensu lato 114
Tremella juscosuccinea C.-J. Chen 124
Tremellagriseobrunnea C.-J. Chen 128
Tremella mycophaga Martin 131
Tremella neofoliacea C.-J. Chen 135
Tremella vasifera C.-J. Chen 143
3.1.5. Aurantia group 150
Tremella aurantia Schw.: Fr. 152
Tremella australiensis Lloyd 159
Tremella encephala Pers.: Bref 166
Tremella giraffa C.-J. Chen 173
Tremella microspora Lloyd 179
3.1.6. The genus Sirobasidium 187
Sirobasidium magnum Boed. 189
3.2. Ultrastructure 194
3.2.1. Septal pore ultrastructure 194
3.2.2. Haustoria-host hyphae interaction 194
3.3. Morphological phylogenetic analysis 198
3.3.1. Morphological matrix 198
3.3.2. Morphological phylogeny 198
3.4. Molecular analysis 203
3.4.1. rDNA sequences 203
3.4.2. rDNA phylogenetic analysis 204
3.4.2.1. Phylogeny of the large subunit (LSU) rDNA 204
3.4.2.2. Phylogeny of the internal transcribed spacer (ITS) rDNA 204
4. Discussions 208
4.1. Interaction of haustona-host hyphae 208
4.2. Comparison of morphological and molecular phylogeny 209
4.2.1. Mesenterica group 209
4.2.2. Fuciformis group 209
4.2.3. Indecorata group 210
4.2.4. Foliacea group 211
4.2.5. Aurantia group 211
4.2.6. The genus Sirobasidium 212
5. Summary 213
6. Literature 214
7. Index 223

8. Abbreviation 225