Original paper
EDTA in algal culture media
Dufková, Věra
Algological Studies/Archiv für Hydrobiologie, Supplement Volumes No. 67 (1984), p. 479 - 492
24 references
published: Dec 12, 1984
DOI: 10.1127/algol_stud/67/1984/479
ArtNo. ESP142009500033, Price: 29.00 €
Abstract
The action of EDTA in batch cultures of Scenedesmus quadricauda grown under continuous illumination with either fluorescent tubes or high-pressure sodium discharge lamps was investigated. The effects of various concentrations and ratios of macroelements, microelements and EDTA in the nutrient solution on the growth characteristics of the algal suspensions were evaluated. Higher concentrations of EDTA (Na2EDTA·2H2O: 400 mg·1-1), when in surplus over trace elements in the nutrient solution, inhibited cell division, chlorophyll synthesis and the production of algal biomass, especially in the earlier phase of algal growth. No negative influence of the higher concentration of EDTA was observed when the concentration of trace elements in the nutrient solution was increased correspondingly to the increased EDTA concentration. EDTA depletion from the solutions was observed in the experiments where a surplus of EDTA was accompanied by a corresponding surplus of trace elements. The highest decrease in EDTA concentration corresponded to the highest rate of cell division. The influence of light sources on the decomposition of EDTA in the algal nutrient solution was also studied, but no decomposition of EDTA by the light sources was proved.
Keywords
Algae • Scenedesmus • batch cultivation • EDTA • light sources • growth characterisctics • cell division inhibition • chlorophyll inhibition • dry mass inhibition • magnesium