Beitrag
Laminarinase activity in the rabbitfish, Siganus canaliculatus (PARK)
Sabapathy, Uma
Archiv für Hydrobiologie Volume 136 Number 1 (1996), p. 127 - 134
19 Literaturangaben
veröffentlicht: Feb 22, 1996
DOI: 10.1127/archiv-hydrobiol/136/1996/127
ArtNo. ESP141013601006, Preis: 29.00 €
Abstract
High laminarinase activity was observed in the digestive tract of the rabbitfish, Siganus canaliculatus (PARK). Crude extracts of the intestine were used to examine some properties of the laminarinase using laminarin as substrate. At 37° C, maximum activity was observed at pH 6.0. The optimum temperature was 45° C. The activation energy, Ea, was estimated to be 2960 cal/mol. The Lineweaver-Burk plot was used to estimate the Michaelis constant, Km, -27.17 mg/ml.
Schlagworte
laminarinase • rabbitfisch • digestive tract • substrate • Lineweaver-Burk