Contribution
ZcGSTe1 regulates oxidative stress during vitellogenesis in Zeugodacus cucurbitae
Liu, Chuan-Lian; Ma, Meng; Yi, Jun-Chen; Chen, Dong; Wang, Yun; Wang, Jin-Jun; Wei, Dong
Entomologia Generalis Volume 44 Number 6 (2024), p. 1547 - 1556
publié: Dec 12, 2024
publication en ligne: Dec 4, 2024
manuscrit accepté: Oct 5, 2024
révision final du manuscrit reçu: Sep 30, 2024
révision du manuscrit demandée: Sep 9, 2024
manuscrit reçu: Jul 2, 2024
DOI: 10.1127/entomologia/2024/2816
ArtNo. ESP146004406016, Prix: 29.00 €
Abstract
The melon fly, Zeugodacus cucurbitae Coquillett, is a significant quarantine pest belonging to the Diptera, known for its extended oviposition period, which causes significant economic damage to fruits and vegetables. Glutathione S-transferases (GSTs) are essential enzymes involved in insect pesticide detoxification and antioxidant enzyme activities. In this study, the GSTe1 gene was identified as being specifically expressed in the ovaries of melon fly. Heterologous expression demonstrated that the ZcGSTe1 recombinant protein catalyzed substrates 1-choro-2, 4-dinitrobenzene (CDNB) and peroxidase, with specific activities of 113.09 U/mg protein and 45.87 U/mg protein, respectively. The result of fluorescence in situ hybridization (FISH) analysis revealed that ZcGSTe1 was mainly enriched in oocytes. Using CRISPR/Cas9-mediated genome editing, a ZcGSTe1 knockout strain with a 28 bp deletion was created. Compared to wild-type females, homozygous mutants showed a 21% reduction in mating rate at 60 min, a 32% decrease in egg-laying over five days, and a 15.65% reduction in ovarian size of six-day-old females. Additionally, oocytes from five-day-old mutant females showed increased reactive oxygen species (ROS) levels. These findings suggest that ZcGSTe1 may play a role in ovarian development by regulating ROS levels through its antioxidant activity.
Mots-clefs
melon fly •
ovary •
CRISPR/Cas9 •
glutathione •
S-transferase •
reactive oxygen species