leaf •
surface •
woddy plant •
broadleaf •
Central Europe •
Blatt •
Oberfläche •
Laubgehölz •
Laub •
Mitteleuropa
Description of content
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Leaves usually suffice to identify the woody plants they belong to, if
the leaf samples are large enough and well preserved. In certain
fields of applied biology (e.g. forensic sciences, palaeobiology)
small fragments constitute the bulk of the material for
investigation. Until now, it was next to impossible to identify the
corresponding woody plant down to the species level from these
fragments.
Only non-destructive methods were considered for examination in order
to leave the samples unaltered, e.g. for their use as forensic
evidence. Scanning electron microscopy (SEM) has proved an
appropriate method. Without time-consuming sample preparation it
allows investigation of leaf surface micromorphology (indumentum,
shape and number of stomata, undulation of anticlinal boundaries,
sculpture of outer periclinal walls, etc.). These data may then be
used to determine the species of the corresponding woody plant. The
wealth of information hidden in leaf surfaces has in the past been
used to clarify relationships within narrow taxa but has never been
applied to species discrimination of larger groups as heterogeneous as
woody plants.
With leaves of 300 species, this atlas comprises all important
broadleaves of Central Europe. As the atlas also considers frequently
cultivated economic and ornamental plants besides indigenous species,
its usefulness extends far beyond Central Europe.
All important structures of abaxial and adaxial leaf surfaces are
painstakingly described applying a uniform terminology. 250 species are
illustrated on plates with some 1900 SEM micrographs depicting
standardized surveys as well as significant details.
Computer-aided processing of the leaf-structure data using the
DELTA system
resulted in standardized species descriptions and a key which allows for a
separation and thus identification of almost all the species
investigated.
For the first time, leaf surface micromorphology of broadleaves is described
and depicted in a standardized format and used for species identification. As
all leaf-surface characteristics can be determined on tiny surface moieties,
even the exact identification of leaf fragments is possible.
Beschreibung
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Laubgehölze lassen sich in der Regel anhand ihrer Blätter bestimmen,
sofern diese mehr oder minder vollständig erhalten sind. In manchen
Bereichen der Angewandten Biologie (z.B. Kriminaltechnik, Paläobiologie)
besteht das zu untersuchende Material aus kleinen Fragmenten, die
bisher nicht bestimmbar waren.
Für die Untersuchung kommen nur zerstörungsfreie Untersuchungsmethoden in
Betracht, damit die Objekte unverändert erhalten bleiben, um beispielsweise
in der naturwissenschaftlichen Kriminalistik als Beweismittel zu dienen.
Eine solche Methode ist die Rasterelektronenmikroskopie. Ohne großen
präparativen Aufwand erlaubt sie, die zahlreichen Merkmale der
Mikromorphologie der Blattoberflächen (Behaarungstypen, Form und Dichte der
Stomata, Verlauf der Zellgrenzen, Skulpturierung von Zelloberflächen, usw.)
zu analysieren und als Kriterien für eine Bestimmung nutzbar zu machen. Der
Informationsgehalt dieser Strukturen ist zwar schon verschiedentlich für die
Klärung systematischer Probleme innerhalb enger Verwandschaftskreise genutzt
worden, aber noch nie zur Artbestimmung in größerem Maßstab und für eine
heterogene Gruppe wie die Gehölze zur Anwendung gekommen.
Der vorliegende Atlas enthält mit 300 Arten alle in Mitteleuropa wichtigen
Laubgehölze. Neben den heimischen Arten berücksichtigt er auch die häufiger
kultivierten Zier- und Nutzpflanzen und ist daher weit über Mitteleuropa
hinaus von Nutzen.
Alle wichtigen Strukturen von Blattunter- und -oberseite werden nach einem
einheitlichen Muster minutiös beschrieben. 250 Arten werden darüber hinaus
auf Bildtafeln mit insgesamt fast 1900 rasterelektronenmikroskopischen
Aufnahmen illustriert. Neben standardisierten Übersichtsphotos sind alle
wichtigen Strukturen im Detail abgebildet.
Die große Menge an Strukturinformationen wurde mit dem EDV-System DELTA
sowohl zu einheitlichen Artbeschreibungen als auch zu einem
Bestimmungsschlüssel verarbeitet, mit dem sich fast alle untersuchten Arten
voneinander trennen und somit erkennen lassen.
Erstmals wird die Mikromorphologie der Blattoberflächen von Laubgehölzen in
einem einheitlichen Format beschrieben und abgebildet sowie für eine
Bestimmung der Arten nutzbar gemacht. Da alle Merkmale an winzigen
Blattausschnitten erkannt werden können, ist damit eine exakte Bestimmung
auch an kleinsten Blattfragmenten möglich.
Das Buch richtet sich an alle Botaniker, Forstleute, Archäologen,
kurzum alle, die Laubgehölze anhand kleinster Reste identifizieren
wollen.
Review: "Folia Geobotanica" 34/2, 1999, p. 286
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The book is a unique atlas of leaf surfaces of Central European woody
plants accompanied by keys for their identification. Publication of
the book was promoted by the need for such an atlas in
criminology. However, it could be a very useful reference book for
pharmaceutical biologists, paleobotanists, palynologists and also for
zoologists investigating samples from the alimentary tract or from
excrement in order to determine plants which had been ingested.
The method used for obtaining images of leaf surfaces complies with
the original intention to prepare a reference atlas for forensic
examinations. The illustrations used are scanning-electron-microscopic
images of air-dried specimens of leaf fragments, thus allowing a fast
processing of samples without time-consuming preparations. To generate
standardised descriptions of species-specific leaf surfaces and the
keys the data were processed using the computer-aided DELTA system
(Description Language for Taxonomy). The generated keys also included
in the book allow identification independently of the approach to the
DELTA system. The keys allow a fast identification of leaf surfaces of
301 woody species, only nine species of which cannot be definitely
separated.
The book consists of three main units. The first includes an
introductory text to the following parts and consists of the preface,
the introduction, the material and the methods, the character list for
using the DELTA system, the glossary with remarks on character
weighting, and the keys for manual identification. The main body of
the book is a standardized atlas of leaf surfaces. Photographically
documented species are presented alphabetically at the beginning of
this unit. A double page is devoted to one species and has a standard
arrangement: The first page gives the images of adaxial and abaxial
surfaces, including the total view on the surface (first row) then the
detail of a standard area, and the detail of the outer periclinal wall
of the stoma(ta) (second row) and details of additional epidermal
structures, such as hairs and domatia, if present (third row). The
second page gives a written description of the characters of the
particular leaf surfaces for a given species consisting of the
description of the macro-morphology, the abaxial surface, the adaxial
surface, the indumentum, and the habitat of the species. The atlas
part continues with the alphabetically arranged remaining woody
species, whose leaf surfaces were not illustrated in the first
part. This list corresponds in its arrangement to the second page of
the species accounts of the first part of the atlas, i.e. it includes
the same descriptive sections. The last part of the atlas includes
references and an index with German, English and Latin names of
included species.
The big advantages of the book are firstly its bilingual text in
German and English, and secondly the unusual format, which is very
advantageous for this purpose. The book could be of use not only for
the above-mentioned specialists, but also as a reference book for the
study of plant anatomy, as it gives the whole range of variability of
leaf surfaces of woody plants. It is important to add that the atlas
does not contain coniferous species (e.g. genera Picea and Pinus).
Jana Albrechtóva
"Folia Geobotanica" 34/2, 1999, p. 286
Inhaltsverzeichnis
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Vorwort A 2
Einleitung A 4
Material und Methoden A 6
Merkmale A 7
Glossar (mit Angaben zur Merkmalsbewertung) A 19
Schlüssel A 26
Atlas 1
Literatur 553
Index 555
Table of Contents
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Preface A 2
Introduction A 4
Materials and Methods A 6
Characters A 7
Glossary (with remarks on character weighing) A 19
Keys A 26
Atlas 1
References 553
Index 555