Original paper
Two glutathione S-transferases, PaGSTδ1 and PaGSTω1 are involved in pyrethroids detoxification in the American cockroach, Periplaneta americana
Sun, Hong; Li, Yifan; Li, Xinyu; Wang, Pei; Wei, Chunqi; Li, Ruichi; Fan, Qiqi; Liu, Jiyuan; Zhang, Yalin
Entomologia Generalis Volume 43 Number 3 (2023), p. 659 - 668
published: Sep 6, 2023
published online: Aug 8, 2023
manuscript accepted: Jun 11, 2023
final revised version received: Jun 7, 2023
manuscript revision requested: Apr 16, 2023
manuscript received: Feb 15, 2023
DOI: 10.1127/entomologia/2023/2006
ArtNo. ESP146004303016, Price: 29.00 €
Abstract
The American cockroach, Periplaneta americana (L.), is a notorious pest. Pyrethroids are one of the most applied insecticides in controlling the pest. Although previous work indicated that glutathione S-transferases (GSTs) detoxified pyrethroids, the roles of GSTs in the pyrethroids detoxification in P. americana remained unknown. Herein, two P. americana GSTs, PaGSTδ1 and PaGSTω1, highly expressed in the midgut and fat body were identified. Their expression levels show significant and immediate increase after exposure to four pyrethroids, including bifenthrin, cypermethrin, λ-cyhalothrin and β-cyfluthrin, with PaGSTδ1 mostly expressed from bifenthrin exposure. RNAi-mediated knockdown of PaGSTδ1 and PaGSTω1 increased the sensitivity of the P. americana to these four pyrethroids, especially to bifenthrin. Both recombinant PaGSTδ1 and PaGSTω1 proteins can bind pyrethroids, with in vitro metabolism confirmed for bifenthrin and λ-cyhalothrin for recombinant PaGSTδ1. This study identifies the role of PaGSTδ1 and PaGSTω1 in the detoxification of pyrethroids, and suggests the necessity of rotation between bifenthrin and other insecticides to avoid resistance in P. americana control.
Keywords
toxicity • GSTs • bifenthrin • sensitivity • insecticide management