Original paper
Disruption of sex pheromone biosynthesis in Plutella xylostella by sublethal avermectin exposure: a multigenerational perspective
Chang, Zhu; Yao, Shuangyan; Li, Zhen; Lei, Quanxing; Liu, Xiaoguang; Du, Mengfang; Na, Risong; Zhang, Yunhui; An, Shiheng
Entomologia Generalis Volume 45 Number 5 (2025), p. 1395 - 1406
published: Nov 18, 2025
published online: Sep 12, 2025
manuscript accepted: Jul 15, 2025
final revised version received: Jun 24, 2025
manuscript revision requested: May 22, 2025
manuscript received: Apr 7, 2025
ArtNo. ESP146004505014, Price: 29.00 €
Abstract
The diamondback moth (Plutella xylostella) represents a formidable challenge in agriculture due to its rapid reproductive cycle and increasing resistance to conventional insecticides. This study investigates the regulation of sex pheromone biosynthesis in P. xylostella, focusing on the role of the neuropeptide pheromone biosynthesis activating neuropeptide (PBAN) and its downstream signaling pathways. Through transcriptomic analysis, we identified key genes involved in pheromone production, including the PBAN receptor (PBANR), calcineurin (CaN), and acetyl-CoA carboxylase (ACC). Further investigation was carried out to examine the effects of avermectin, a naturally derived insecticide, on both the pheromone biosynthesis and mating success of P. xylostella. Our findings reveal that sublethal doses of avermectin significantly disrupt pheromone production, caused by a reduction in extracellular calcium influx, as well as decreased activity of enzymatic pathways crucial for pheromone synthesis. Additionally, the mating success of both F0 and F1 generations was adversely affected, indicating transgenerational impacts of chemical exposure. This study provides new insights into the biochemical pathways regulating sex pheromone production in P. xylostella and highlights the intricate effects of avermectin on pest behavior and population dynamics, contributing to the development of sustainable agricultural practices.
Keywords
Diamondback moth • PBAN • Life table • mating disruption • neuropeptide signaling • mating behavior • pesticide