Original paper
Gene-targeted RNAi enhances biocontrol and insecticide synergy against the rice pest Sogatella furcifera
Yan, Xin; Yan, ChangMei; Pu, YongKun; Jiang, ZhaoChun; Yang, Hong; Smagghe, Guy
published online: Jun 2, 2026
manuscript accepted: Feb 23, 2026
final revised version received: Feb 11, 2026
manuscript revision requested: Oct 9, 2025
manuscript received: Sep 2, 2025
Open Access (paper may be downloaded free of charge)
Abstract
The white-backed planthopper, Sogatella furcifera, is a major rice pest in Asia, and increasing insecticide resistance underscores the need for novel control strategies. In this study, we developed an RNA interference (RNAi)-based approach targeting four nuclear receptor genes, -SfERR, SfSVP, SfKNI, and SfβFTZ-F1-that regulate molting and development in S. furcifera. These genes were highly expressed during the 5th-instar nymph stage and were upregulated by 20-hydroxyecdysone (20E). RNAi-mediated silencing disrupted 20E signaling and chitin metabolism, leading to decreased hormone titers, lower chitin content, suppression of biosynthesis-related genes, and ultimately resulting in lethal molting impairments. Furthermore, combining RNAi with either entomopathogenic fungi (Beauveria bassiana and Metarhizium anisopliae) or chemical insecticides (thiamethoxam and buprofezin) significantly enhanced mortality compared to individual treatments, demonstrating strong synergistic pest control. This integrated RNAi strategy mitigates chemical overuse and resistance development, providing an effective and ecologically sustainable tool for integrated pest management in rice.
Keywords
nuclear receptor • RNA interference • molting • pest management • white-backed planthopper • chitin metabolism • insecticide resistance management