Original paper
An improved direct viable count method for aquatic bacteria
Kogure, Kazuhiro; Simidu, Usio; Taga, Nobuo
Archiv für Hydrobiologie Volume 102 Number 1 (1984), p. 117 - 122
18 references
published: Nov 14, 1984
DOI: 10.1127/archiv-hydrobiol/102/1984/117
ArtNo. ESP141010201008, Price: 29.00 €
Abstract
The direct viable count (DVC) method was modified so as to differentiate the living aquatic bacteria more clearly and easily. In order to cover variety bacterial groups, 3 antibiotics (piromidic acid, pipemidic acid and nalidixic acid) which inhibit the bacterial division during the incubation, were used. After adding these antibiotics and yeast extract, seawater samples were incubated for 8 hours at 20 °C, followed by the fluorescent microscopic observation and counting of living bacterial cells. The results obtained by applying the improved method to the coastal area in Japan were almost the same as those by the original method, which uses only nalidixic acid and 6 hours incubation.
Keywords
direct viable count method • acquatic bacteria • antibiotics • inhibit • Japan