Contribution
A tachykinin receptor affects starvation tolerance and feeding behavior in the fall webworm, Hyphantria cunea
Sun, Li-li; Ma, He-ting; Wu, Hong-qu; Wu, Shao-ping; Yan, Li-qiong; Zhang, Chen-shu; Nur, Faidah Arina; Wang, Zhi-ying; Cao, Chuan-wang
Entomologia Generalis Volume 42 Number 4 (2022), p. 621 - 630
publié: Jul 11, 2022
publication en ligne: Feb 4, 2022
manuscrit accepté: Nov 25, 2021
revision du manuscrit reçu: Sep 28, 2021
révision du manuscrit demandée: Jun 30, 2021
manuscrit reçu: May 14, 2021
DOI: 10.1127/entomologia/2022/1366
ArtNo. ESP146004204013, Prix: 29.00 €
Abstract
The insect tachykinin-related peptide (TRP), as an ortholog of tachykinin in vertebrates, regulates diverse physiological processes, including stress resistance, locomotion, aggression, lipid metabolism, and myotropic activity. In the present study, the functions of TRP and its receptor (HcTRPR) were investigated in the fall webworm, Hyphantria cunea. The results showed that HcTRPR, a typical G protein-coupled receptor, could be activated by the putative HcTRP mature peptides expressed in HEK293T cells. The effective concentrations (EC50) of TRP3 and TRP6 were 26.35 nM and 8.82 nM, respectively. HcTRP and HcTRPR were expressed in the head, midgut, hindgut, and testis of every developmental stage, and were especially highly expressed at the larval stage. To investigate the roles of HcTRP and HcTRPR in starvation stress and food intake, H. cunea larvae were assessed for the inhibitory effects of HcTRP and HcTRPR on food intake and susceptibility to starvation resistance, followed by determination of insulin-like peptide mRNA expression levels. The results showed that TRP signaling is involved in the regulation of starvation in H. cunea, and that TRP interacts with insulin signaling in response to starvation stress. Taken together, the effects of TRP/TRPR signaling on the modulation of the starvation stress in H. cunea were demonstrated. The results suggested that components of the neuropeptide signaling system could be exploited as potential targets for pest control.
Mots-clefs
Tachykinin-related peptide • RNA interference • stress • signaling system • insulin signalling