Original paper
The green alga Chlamydomonas reinhardtii: bioactivator of nitrosoamines
Vlčková, Viera; Podstavková, Svetlana; Slaninová, Miroslava; Miadoková, Eva; Vlček, Daniel
Algological Studies/Archiv für Hydrobiologie, Supplement Volumes No. 100 (2000), p. 181 - 193
33 references
published: Dec 20, 2000
DOI: 10.1127/algol_stud/100/2000/181
ArtNo. ESP142013600011, Price: 29.00 €
Abstract
The unicellular green alga Chlamydomonas reinhardtii can be used as an activating system in an algal cell/microbe coincubation assay that complements those demonstrated in mammals and higher plants. It is useful for detection of environmental mutagens and conversion of promutagens, occurring mainly in aquatic environments. Previously we used the green alga Chlamydomonas reinhardtii as the activating organism and the bacterium Salmonella typhimurium and yeast Saccharomyces cerevisiae as genetic indicators for activation of mono- and polycyclic aromatic amines. In this work we used the C. reinhardtii/microbe coincubation assay for N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) activation. The different responses of the algal wild-type strain and repair-deficient strains to the toxic effects of NDMA and NDEA were observed. The recombination-deficient strain was the most sensitive to both compounds, indicating that recombination process plays an important role in DNA repair of nitrosoamines induced damages. NDEA was more toxic than NDMA to both intact algal and yeast cells, and in the algal cell/microbe coincubation assays. Of the two nitrosoamines tested, NDEA was activated both in the algal cell/S. typhimurium and the algal cell/S. cerevisiae coincubation assays, but NDMA was only weekly activated by the algal cell/S. cerevisiae coincubation assay.
Keywords
Chlamydomonas reinhardtii • Saccharomyces cerevisiae • Salmonella typhimurium • promutagen • algal activation • algal cell/microbe coincubation assay