Original paper
Oral RNAi assays in Henosepilachna vigintioctopunctata suggest HvSec23 and HvSar1 as promising molecular targets for pest control
Guo, Mujuan; Nanda, Satyabrata; Yang, Chunxiao; Li, Zhaoyang; Liu, Junna; Gao, Ran; Zhang, Youjun; Zhou, Xuguo; Pan, Huipeng
Entomologia Generalis Volume 43 Number 1 (2023), p. 147 - 155
published: Mar 22, 2023
published online: Dec 19, 2022
manuscript accepted: Nov 10, 2022
manuscript revision received: Aug 8, 2022
manuscript revision requested: Jul 19, 2022
manuscript received: Jun 21, 2022
DOI: 10.1127/entomologia/2023/1712
ArtNo. ESP146004301013, Price: 29.00 €
Abstract
The coat protein II (COPII) complex is a class of coated vesicles involved in protein transport from the endoplasmic reticulum to the Golgi apparatus, serving a variety of important roles in animal growth and development. In this study, we selected three COPII genes, HvSec23, HvSec24, and HvSar1 to explore the RNAi silencing effect on Henosepilachna vigintioctopunctata (HVig), a dreadful solanaceous crop pest, using the dietary RNA interference (RNAi) technique. The results showed that HvSec23, HvSec24, and HvSar1 had a significant lethal effect on the 1st and 3rd instars of HVig. In addition, oral administration of the bacterially expressed dsHvSec23 and dsHvSar1 caused high mortality and feeding inhibition in both larvae and adults, whereas dsHvSec24 only had a significant effect on the larvae. Moreover, the predatory ladybeetle Propylaea japonica was tested for evaluating the non-target effects of dsHvSec23 through oral and injection RNAi. Results confirmed that gene expression of PjSec23 was significantly suppressed by injection RNAi, but not oral RNAi. Overall, our results revealed that HvSec23 and HvSar1 could be used as promising molecular targets for controlling HVig.
Keywords
coat protein II complex •
RNA interference •
mortality •
Propylaea japonica
•
risk assessment •
non-target effect